pes-1::nls-gfp transcriptional fusion [Expr7325]

pes-1 wormbase gene report


NOTE: YouTube "thumbnails" are just an appetizer. Each genotype has several 4D movies that can be viewed interactively at full resolution.


3 Slice4D movies

pes-1%NLS%gfp_0av_slc.mov
pes-1%NLS%gfp_1av_slc.mov
pes-1%NLS%gfp_2av_slc.mov


3 Stereo4D movies

pes-1%NLS%gfp_0av_pryx.mov (also viewable in ®© RedCyanStereo)
pes-1%NLS%gfp_1av_pryx.mov (also viewable in ®© RedCyanStereo)
pes-1%NLS%gfp_2av_pryx.mov (also viewable in ®© RedCyanStereo)

1 comment:

wmohler said...

Movies are made from hanging embryo mounts (random orientation for each embryo).

All three recorded embryos fail to develop fully through morphogenesis. Annotated time-stamps are very poor approximations for this set of movies, most likely very wrong.

Very tight nuclear localization of GFP in expressing cells

Expression begins in 8-12 cells
~3-4 cell cycles before the onset of morphogenesis. Expression fading away from peak by the time embryonic movement is due to occur.