pal-1 wormbase gene report
NOTE: YouTube "thumbnails" are just an appetizer. Each genotype has several 4D movies that can be viewed interactively at full resolution.
3 Slice4D movies
3 Stereo4D movies
pal-1%yfp_0ao_pryx.mov (also viewable in ®© RedCyanStereo)
pal-1%yfp_0ar_pryx.mov (also viewable in ®© RedCyanStereo)
pal-1%yfp_2ar_pryx.mov (also viewable in ®© RedCyanStereo)
Movies made with hanging-embryo mounts (random DV/RL orientation for each embryo).
Integrated transgene array.
All three movies have the embryo misoriented with anterior to the right.
Earliest expression in 4 C-derived cells within an hour or two of fertilization. YFP remains very bright in posterior/dorsal hyp7 precursors until elongation begins. Cell fusion and perhaps decreased expression rapidly diminish this signal.
A variety of other lineages also express, with resulting fluorescence in a wide selection of tissues.
Several beautiful independently migrating cells are nicely highlighted and visible along much of their routes during morphogenesis.
0Jar = dorsolateral view
2Jar = ventral view
0Jao = dorsolateral view, very high pre-adjusted brightness setting
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