pal-1::yfp transcriptional fusion [Expr7324]

pal-1 wormbase gene report

NOTE: YouTube "thumbnails" are just an appetizer. Each genotype has several 4D movies that can be viewed interactively at full resolution.

3 Slice4D movies

3 Stereo4D movies (also viewable in ®© RedCyanStereo) (also viewable in ®© RedCyanStereo) (also viewable in ®© RedCyanStereo)

1 comment:

wmohler said...

Movies made with hanging-embryo mounts (random DV/RL orientation for each embryo).

Integrated transgene array.

All three movies have the embryo misoriented with anterior to the right.

Earliest expression in 4 C-derived cells within an hour or two of fertilization. YFP remains very bright in posterior/dorsal hyp7 precursors until elongation begins. Cell fusion and perhaps decreased expression rapidly diminish this signal.
A variety of other lineages also express, with resulting fluorescence in a wide selection of tissues.

Several beautiful independently migrating cells are nicely highlighted and visible along much of their routes during morphogenesis.

0Jar = dorsolateral view
2Jar = ventral view
0Jao = dorsolateral view, very high pre-adjusted brightness setting