tkt-1::gfp transcriptional fusion [Expr7305]

tkt-1/F01G10.1 wormbase gene report


NOTE: YouTube "thumbnails" are just an appetizer. Each genotype has several 4D movies that can be viewed interactively at full resolution.


3 Slice4D movies

F01G10.1%gfp_0am_slc.mov
F01G10.1%gfp_1am_slc.mov
F01G10.1%gfp_2am_slc.mov


3 Stereo4D movies

F01G10.1%gfp_0am_pryx.mov (also viewable in ®© RedCyanStereo)
F01G10.1%gfp_1am_pryx.mov (also viewable in ®© RedCyanStereo)
F01G10.1%gfp_2am_pryx.mov (also viewable in ®© RedCyanStereo)

1 comment:

wmohler said...

Movies made with hanging-embryo mounts (random DV/RL orientation for each embryo).

Integrated transgene array.

Earliest expression ~160 minutes after fertilization, in four E-lineage gut precursors. GFP becomes very bright in all gut cells as the embryo approaches and undergoes morphogenesis.

In 0Jam, starting ~330 minutes, expression begins at a low-moderate level in dorsolateral muscle cells and perhaps in dorsal hyp7 precursors. Not seen in other two movies. But each shows two small cells in the head(?) expressing separate from the gut group.

0Jam = dorsal view
1Jam = dorsal view
2Jam = dorsolateral view