arf-3 wormbase gene report
NOTE: YouTube "thumbnails" are just an appetizer. Each genotype has several 4D movies that can be viewed interactively at full resolution.
3 Slice4D movies
arf-1%gfp_0al_slc.mov
arf-1%gfp_1al_slc.mov
arf-1%gfp_2al_slc.mov
3 Stereo4D movies
arf-1%gfp_0al_pryx.mov (also viewable in ®© RedCyanStereo)
arf-1%gfp_1al_pryx.mov (also viewable in ®© RedCyanStereo)
arf-1%gfp_2al_pryx.mov (also viewable in ®© RedCyanStereo)
3 comments:
Expression has already begun by ~180 minutes post fertilization.
All three movies are hanging-embryo mounts, with random DV/LR orientation.
0Jal = Ventrolateral view beginning after ventral enclosure shortly before movement.
1Jal = Dorsal view just after onset of expression. Rather faint. Appears to be only seam cells that express strongly.
2Jal = Quite different pattern than 1Jal with mostly AB lineage hypodermal/epidermal expression. C-derived hyp cells are conspicuously un-labeled before dorsal hyp7 cell fusions. GFP becomes especially prominent in seam cells after morphogenesis.
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