aff-1::gfp transcriptional fusion [Expr7301]

aff-1 wormbase gene report

NOTE: YouTube "thumbnails" are just an appetizer. Each genotype has several 4D movies that can be viewed interactively at full resolution.

5 Slice4D movies

5 Stereo4D movies (also viewable in ®© RedCyanStereo) (also viewable in ®© RedCyanStereo) (also viewable in ®© RedCyanStereo) (also viewable in ®© RedCyanStereo) (also viewable in ®© RedCyanStereo)


wmohler said...

All five embryos show a set of three cells that fuse and extend processes to form a "ringstand" structure in the anterior of the embryo: processes join to form a tiny annular structure near the bucal opening. Embryos and also show small epidermal cells in the head.

The interesting triad of cells may likely be the m1 pharyngeal syncytium.

In embryo, it appears that three additional cells join the first three, resulting in a structure with 6 cell bodies. It is possible that each of the latter three cells fuses with the main syncytium. However, in the other four embryos, the main triad persists with only three cell bodies highlighted. For this reason, it seems that additional cell bodies in 3Jbh may form a separate syncytium.

wmohler said...

In embryo 0Jbh, there also appear to be more than one tri-radial "propeller" or ring-stand structure. This embryo also clearly shows the extension and fusion of two hyp5 cells of the epidermis to form a toroidal syncytium around the head.

wmohler said...

Extrachromosomal array. Hanging embryo mount (random DV/LR orientation for each embryo).

wmohler said...

The "ringstand"-like triads of cells are most likely arcade cells, but might also be pm1 muscles of the pharynx.

Their embryonic morphology most closely matches the adult morphology of the arcade cells, which extend forward to form fused toroids anterior to the pharynx procorpus.