mab-21::yfp transcriptional fusion [Expr7321]

mab-21 wormbase gene report

NOTE: YouTube "thumbnails" are just an appetizer. Each genotype has several 4D movies that can be viewed interactively at full resolution.

3 Slice4D movies

3 Stereo4D movies (also viewable in ®© RedCyanStereo) (also viewable in ®© RedCyanStereo) (also viewable in ®© RedCyanStereo)

1 comment:

wmohler said...

Movies made with hanging-embryo mounts (random DV/RL orientation for each embryo).

Integrated transgene array.

Expression begins ~230 min post fertilization in all 3 movies. Fluorescence very specific to fusion-fated hypodermal/epidermal precursor cells of hyp7+hyp6 syncytia, including both dorsal and some ventral cells. Remarkable match to expression pattern of the eff-1 gene, which encodes a cellular fusogen.

All three movies have the embryo misoriented with anterior to the right.

0Jbj = dorsolateral view
1jaa = dorsal view
3Jbj = lateral view